shrna oligonucleotide sequences Search Results


oligonucleotides containing hoxc8 shrna sequences  (Biosettia)
90
Biosettia oligonucleotides containing hoxc8 shrna sequences
Oligonucleotides Containing Hoxc8 Shrna Sequences, supplied by Biosettia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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shrna oligonucleotide sequences targeting mouse irf8 gene mrna  (SunBio Inc)
90
SunBio Inc shrna oligonucleotide sequences targeting mouse irf8 gene mrna
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Shrna Oligonucleotide Sequences Targeting Mouse Irf8 Gene Mrna, supplied by SunBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c1qtnf6-targeting short hairpin rna (shrna) oligonucleotides sequences tgtgtgagatccctatggt  (Genechem)
90
Genechem c1qtnf6-targeting short hairpin rna (shrna) oligonucleotides sequences tgtgtgagatccctatggt
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
C1qtnf6 Targeting Short Hairpin Rna (Shrna) Oligonucleotides Sequences Tgtgtgagatccctatggt, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oligonucleotides encoding shrna sequences  (Genechem)
90
Genechem oligonucleotides encoding shrna sequences
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Oligonucleotides Encoding Shrna Sequences, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna oligonucleotides encoding shrna sequences  (Biosettia)
90
Biosettia dna oligonucleotides encoding shrna sequences
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Dna Oligonucleotides Encoding Shrna Sequences, supplied by Biosettia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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shrna sequence containing oligonucleotides  (Vigene Biosciences)
90
Vigene Biosciences shrna sequence containing oligonucleotides
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Shrna Sequence Containing Oligonucleotides, supplied by Vigene Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oligonucleotides of shrna sequence targeting pk2 (sh-pk2)  (Sangon Biotech)
90
Sangon Biotech oligonucleotides of shrna sequence targeting pk2 (sh-pk2)
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Oligonucleotides Of Shrna Sequence Targeting Pk2 (Sh Pk2), supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lentiviral shrna oligonucleotide sequences  (Obio Technology Corp Ltd)
90
Obio Technology Corp Ltd lentiviral shrna oligonucleotide sequences
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Lentiviral Shrna Oligonucleotide Sequences, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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complementary oligonucleotides contain shrna sequences  (Biocorp Inc)
90
Biocorp Inc complementary oligonucleotides contain shrna sequences
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Complementary Oligonucleotides Contain Shrna Sequences, supplied by Biocorp Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oligonucleotides corresponding to prdm4- and pten-specific small hairpin rna (shrna) sequences  (Shanghai GenePharma)
90
Shanghai GenePharma oligonucleotides corresponding to prdm4- and pten-specific small hairpin rna (shrna) sequences
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Oligonucleotides Corresponding To Prdm4 And Pten Specific Small Hairpin Rna (Shrna) Sequences, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oligonucleotide probes for construction of shrna targeting rar reverse sequence  (Microsynth ag)
90
Microsynth ag oligonucleotide probes for construction of shrna targeting rar reverse sequence
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Oligonucleotide Probes For Construction Of Shrna Targeting Rar Reverse Sequence, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kif18b-targeting short hairpin rna (shrna) oligonucleotide sequences  (GenScript corporation)
90
GenScript corporation kif18b-targeting short hairpin rna (shrna) oligonucleotide sequences
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Kif18b Targeting Short Hairpin Rna (Shrna) Oligonucleotide Sequences, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a ) The effect of AGEs on IRF8 was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative mRNA levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.

Journal: Scientific Reports

Article Title: AGEs Induced Autophagy Impairs Cutaneous Wound Healing via Stimulating Macrophage Polarization to M1 in Diabetes

doi: 10.1038/srep36416

Figure Lengend Snippet: ( a ) The effect of AGEs on IRF8 was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative mRNA levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.

Article Snippet: shRNA oligonucleotide sequences for targeting mouse IRF8 gene mRNA was designed by Sunbio Medical Biotechnology (Shanghai, China).

Techniques: Western Blot, Infection, shRNA, Incubation, Immunofluorescence, Staining, Real-time Polymerase Chain Reaction

AGEs up-regulate expression of IRF8 and induce macrophage autophagy. Enhanced autophagic activity promotes macrophage polarization to M1. Furthermore, increased M1 macrophages result in excessive inflammation, which leads to impaired wound healing. Together, the mechanism of IRF8-autophagy-M1 polarization is critical for AGEs-induced autophagy impaired wound healing in diabetes.

Journal: Scientific Reports

Article Title: AGEs Induced Autophagy Impairs Cutaneous Wound Healing via Stimulating Macrophage Polarization to M1 in Diabetes

doi: 10.1038/srep36416

Figure Lengend Snippet: AGEs up-regulate expression of IRF8 and induce macrophage autophagy. Enhanced autophagic activity promotes macrophage polarization to M1. Furthermore, increased M1 macrophages result in excessive inflammation, which leads to impaired wound healing. Together, the mechanism of IRF8-autophagy-M1 polarization is critical for AGEs-induced autophagy impaired wound healing in diabetes.

Article Snippet: shRNA oligonucleotide sequences for targeting mouse IRF8 gene mRNA was designed by Sunbio Medical Biotechnology (Shanghai, China).

Techniques: Expressing, Activity Assay